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KMID : 0878220130250010051
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Journal of Sasang Constitutional Medicine
2013 Volume.25 No. 1 p.51 ~ p.61
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Simultaneous Analysis and Anti-obesity Effect of Taeeumin Cheongsimyeonja-tang
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Seo Chang-Seob
Jeong Soo-Jin
Kim Jung-Hoon
Yoo Sae-Room
Shin Hyeun-Kyoo
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Abstract
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Objectives: We performed the simultaneous analysis for three compounds in Cheongsimyeonja-tang (CSYJT) and evaluated its anti-obesity effect.
Methods: The column for separation of three compounds was used Gemini C18 column and maintained at 40℃. The mobile phase for gradient elution consisted of two solvent systems. The analysis was carried out at a flow rate of 1.0 mL/min with PDA detection at 275 nm. The injection volume was 10 μL. 3T3-L1 preadipocytes were differentiated into adipocytes by adding insulin, dexamethasone and 3-isobutyl-1-methylxanthine (IBMX) for 8 days in the absence or presence of CSYJT. Anti-obesity effects of CSYJT were evaluated by Oil Red O staining, glycerol-3-phosphate dehydrogenase (GPDH) activity, triglyceride contents, and leptin production.
Results: Calibration curves were acquired with r2>0.9999. The contents of baicalin, wogonoside and baicalein in CSYJT were 14.54-14.65 mg/g, 5.24-5.27 mg/g and 0.01-0.02 mg/g, respectively.
CSYJT showed inhibitory effect on lipid accumulation and GPDH activity in the differentiated 3T3-L1 cells. Furthermore, CSYJT significantly decreased contents of triglyceride and leptin production in 3T3-L1 adipocytes.
Conclusions: These results will be helpful to improve quality control and anti-obesity effect of Taeeumin CSYJT
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KEYWORD
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Cheongsimyeonja-tang, HPLC-PDA, simultaneous analysis, obesity, 3T3-L1 adipocyte
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